Purification and Properties of Leucyl-tRNA Synthetase from Bakers' Yeast
نویسندگان
چکیده
منابع مشابه
Cytoplasmic Methionyl - tRNA Synthetase from Bakers ’ Yeast
Methionyl-tRNA synthetase has been purified from a yeast strain carrying the MESl structural gene on a high copy number plasmid (pFL1). The purified enzyme is a monomer of M, = 85,000 in contrast to its counterpart from Escherichia coli which is a dimer made up of identical subunits (M, = 76,000; Dardel, F., Fayat, G., and Blanquet, S . (1984) J. Bucteriol. 160,1115-1122). The yeast enzyme was ...
متن کاملPurification and properties of chloroplast leucyl-tRNA synthetase from a higher plant: Phaseolus vulgaris.
The chloroplast leucyl-tRNA synthetase from Phaseolus vulgaris was purified by ammonium sulfate precipitation and chromatography on DEAE-cellulose, hydroxylapatite, and phosphocellulose. Finally, the pure enzyme was obtained after affinity chromatography on Blue Sepharose CL-6B using specific elution with a pure Escherichia coli tRNALeu isoacceptor. The specific activity of chloroplast leucyl-t...
متن کاملPurification and Properties of Bakers' Yeast Trehalase.
Since recent surveys of insect hemolymph (1, 2) have shown that trehalose is the major blood sugar, the hydrolytic enzyme trehalase has also awakened the interest of investigators. A purified preparation of the enzyme from Galleria mellonella (3) and from Phormia regina (4) was recently obtained and some of its properties described. The biosynthesis of trehalose with an enzyme preparation from ...
متن کاملPurification and properties of acetyl coenzyme A synthetase from bakers' yeast.
Acetyl-CoA synthetase, utilized in a coupled reaction system, has been shown to be applicable to the spectrophotometric determination of propionic and methylmalonic acids in biological fluids. The isolation of acetyl-CoA synthetase from yeast is simpler than the purification from mammalian sources. This study also presents some properties of the yeast enzyme and compares it to the more extensiv...
متن کاملPurification and properties of lysyl transfer ribonucleic acid synthetase from bakers' yeast.
Lysyl transfer ribonucleic acid synthetase (L-lysine: tRNA ligase (AMP), EC 6.1.1.6) was pursed to a state of apparent homogeneity from bakers’ yeast. For each of two different preparation procedures, a 250to 500-fold purikation of the enzyme was obtained, and the final purification step yielded two distinct protein components with specific activity values of approximately 0.5 and 1.0 pmole of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44372-x